Candida Feed
- Contributions of Carnitine Acetyltransferases to Intracellular Acetyl Unit Transport in Candida albicans [Enzymology]
Transport of acetyl-CoA between intracellular compartments is mediated by carnitine acetyltransferases (Cats) that reversibly link acetyl units to the carrier molecule carnitine. The genome of the opportunistic pathogenic yeast Candida albicans encodes several (putative) Cats: the peroxisomal and mitochondrial Cat2 isoenzymes encoded by a single gene and the carnitine acetyltransferase homologs Yat1 and Yat2. To determine the contributions of the individual Cats, various carnitine acetyltransferase mutant strains were constructed and subjected to phenotypic and biochemical analyses on different carbon sources. We show that mitochondrial Cat2 is required for the intramitochondrial conversion of acetylcarnitine to acetyl-CoA, which is essential for a functional tricarboxylic acid cycle durin...MedWorm Message: Register for MedMatcha, MedWorm's medical advertising network, and receive $5 free advertising.
- Candida africana and its closest relatives
Candida africana is a recently described opportunistic yeast pathogen that has been linked to vaginal candidiasis. This yeast was first described, in 1995, as atypical chlamydospore-negative Candida albicans strain, and subsequently proposed as a new Candida species on the basis of morphological, biochemical and physiological characteristics clearly different from those of typical C. albicans isolates. Phylogenetic studies based on the comparison of ribosomal DNA sequences demonstrated that C. africana and C. albicans isolates are too closely related to draw any conclusions regarding the status of a new species. Therefore, on the basis of these studies, some authors considered C. africana as a biovar of C. albicans even if genetic differences may be found if additional regions of genomic D... - Evaluation of Antifungal Efficacy of Erbium, Chromium: Yttrium-Scandium-Gallium-Garnet Laser Against Candida albicans
Photomedicine and Laser Surgery , Vol. 0, No. 0. (Source: Photomedicine and Laser Surgery) - Genotypic and phenotypic properties of Candida parapsilosis sensu strictu strains isolated from different geographic regions and body sites
Conclusions: The low number of polymorphic AFLP bands (18 out of 80) obtained for C. parapsilosis isolates is in agreement with the limited sequence variability described for this species. However, when band intensity was included in the analysis, geographical clustering was observed. Expression of virulence factors varied among strains isolated from different geographical regions, with biofilm and proteinase producers more frequently isolated from Hungary and Italy, respectively. (Source: BioMed Central) - Molecular detection and characterisation of fungal heat shock protein 60
Heat shock proteins (Hsp) are highly conserved molecules, which are both constitutively expressed and up-regulated in response to various stress conditions. In particular, fungal Hsp60 can act as immunodominant antigens and facilitate powerful immunological properties. A possible cellular heat shock response was investigated in eight fungi (Aspergillus fumigatus, Aspergillus terreus, Penicillium chrysogenum, Cladosporium cladosporioides, Scedosporium apiospermum, Trichophyton mentagrophytes, Candida albicans and Saccharomyces cerevisiae). Fully automated RNA extraction was followed by quantitative real-time RT-PCR targeting fungus-specific Hsp60 mRNA and sequencing of the amplicon. Levels of temperature-dependent gene expression were evaluated and rates of similarity and identity were comp... - Comparison and evaluation of a novel bioassay and high-performance liquid chromatography for the clinical measurement of serum voriconazole concentrations
The aim of this study was to develop and validate a novel bioassay for determining serum voriconazole (VRC) concentrations and to compare its routine clinical performance with that of high-performance liquid chromatography (HPLC). The biological activity of VRC was measured by a plate diffusion assay using a VRC-hypersusceptible Candida kefyr strain. The bioassay's utility was tested by measuring steady-state VRC concentrations in 100 serum probes from VRC-treated patients. The HPLC system used solvent extraction with hexane : dichloromethane followed by reversed-phase HPLC with ultraviolet detection. The intra-day and inter-day accuracy of the bioassay was (Source: Mycoses)MedWorm Message: Register for MedMatcha, MedWorm's medical advertising network, and receive $5 free advertising.
- Antifungal activity of tri- and tetra-thioureido amino derivatives against different Candida species
This study demonstrated that thioureido derivatives exhibited significant antifungal activity against Candida species and that they can be considered as a very promising bioactive lead compound to develop novel antifungal agents. (Source: Mycoses) - Fluconazole Resistance Analysis in Candida Glabrata Isolates from OPC Patients
Steven A. Mayen, Nathan P. Wiederhold, Tom A. Samuel, William R. Kirkpatrick, Thomas F. Patterson, Spencer W. Redding. University of Texas Health Science Center at San Antonio School of Dentistry (Source: Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics) - Synthesis and antimicrobial activity of Ag(I)-N-heterocyclic carbene complexes derived from benzimidazol-2-ylidene
Novel benzimidazol-2-ylidene carbene complexes of Ag(I) were prepared by interaction of the corresponding benzimidazolium salt with Ag2O in dichloromethane. Their structures were characterized by elemental analyses, 1H-NMR, 13C-NMR and IR spectroscopy techniques. All compounds studied in this work were screened for their in vitro antimicrobial activities against the standard strains: Enterococcus faecalis (ATCC 29212), Staphylococcus aureus (ATCC 29213), Escherichia coli (ATCC 25922), Pseudomonas aeruginosa (ATCC 27853) and the fungi Candida albicans and Candida tropicalis. The new complexes were found to be effective antimicrobial activity against a series of bacteria and fungi. Copyright © 2010 John Wiley & Sons, Ltd. (Source: Applied Organometallic Chemistry) - Epidemiology of Opportunistic Fungal Infections in Latin America
Clinical Infectious Diseases, Volume 0, Issue 0, Page 000, Latest Articles. This review discusses the epidemiology of the most clinically relevant opportunistic fungal infections in Latin America, including candidiasis, cryptococcosis, trichosporonosis, aspergillosis, and fusariosis. The epidemiologic features, including incidence, of some of these mycoses are markedly different in Latin America than they are in other parts of the world. The most consistent epidemiologic data are available for candidemia, with a large prospective study in Brazil reporting an incidence that is 3â to 15âfold higher than that reported in studies from North America and Europe. Species distribution also differs: in Latin America, the most common Candida species (other than Candida albicans) causing bl... - KINETIC RESOLUTION OF (R/S)-PROPRANOLOL (1-isopropylamino-3-(1-naphtoxy)-2-propanolol) CATALYZED BY IMMOBILIZED PREPARATIONS OF CANDIDA ANTARCTICA LIPASE B (CAL-B).
Authors: Barbosa O, Ariza C, Ortiz C, Torres R Enzyme preparations of Candida antarctica-B lipase (CAL-B) - immobilized on Eupergit C and partially modified Eupergit C supports - were tested for kinetic resolution of (R/S)-propanolol, using vinyl acetate as acyl donor, and toluene as solvent organic. The effects of (R/S)-propranolol concentration, vinyl acetate concentration and biocatalyst loading on the esterification and resolution of propranolol were studied. Additionally, different types of immobilized lipase derivatives were also evaluated in terms of its selectivity on kinetic resolution of (R,S)-propranolol. These derivatives showed different enantiomeric ratios (E), with high enantiomeric ratios (E=53) with CAL-B immobilized on Eupergit C supports. PMID: 20667519 [PubMed -...MedWorm Message: Register for MedMatcha, MedWorm's medical advertising network, and receive $5 free advertising.
- Candida rugosa: a possible emerging cause of candidaemia in trauma patients
Discussion Prior to this cluster, C. rugosa had never been identified as a cause of infection at our centre. Due to the retrospective nature of the evaluation of these cases, the source of this possible outbreak could not be traced. Nevertheless, to the best of our knowledge, this is the largest cluster of cases of C. rugosa candidaemia reported from a single institution in the English literature. Content Type Journal ArticleCategory Brief ReportDOI 10.1007/s15010-010-0044-xAuthors B. Behera, All India Institute of Medical Sciences Jai Prakash Narayan Apex Trauma Center New Delhi 110029 IndiaR. I. Singh, All India Institute of Medical Sciences Jai Prakash Narayan Apex Trauma Center New Delhi 110029 IndiaI. Xess, All India Institute of Medical Sciences Jai Prakash Na... - Candida krusei fungemia in an immunocompromised patient.
We present a case of a patient with acute lymphoblastic leukemia who, despite being treated prophylactically with fluconazole, developed disseminated C. krusei. PMID: 20409412 [PubMed - indexed for MEDLINE] (Source: Dermatol Online J) - Correlation between factors associated with the removable partial dentures use and Candida spp. in saliva
Gerodontology 2010; doi: 10.1111/j.1741-2358.2010.00390.x Correlation between factors associated with the removable partial dentures use and Candida spp. in salivaObjectives: To correlate the presence and number of Candida spp. in the saliva of wearers of removable partial dentures retained with precision attachments with the proportion of metal/acrylic resin present in the dentures.Methods: Saliva samples from 40 removable partial denture wearers (test) and one paired sample of individuals, non-wearers of any type of removable denture (control) were collected, seeded, and the colony forming units of Candida counted and identified. The metal/acrylic resin proportion of each denture was quantified, using silicone plates pressed over each denture.Results: Candida spp. was found in the saliva... - Evolutionary divergence in the fungal response
to fluconazole revealed by soft clustering
Conclusions: We have compared the dynamic transcriptional responses of three diverse yeast species to fluconazole treatment using a novel clustering algorithm. This approach revealed significant divergence among regulatory programs associated with fluconazole sensitivity. In future, such approaches might be used to survey a wider range of species, drug concentrations and stimuli to reveal conserved and divergent molecular response pathways. (Source: Genome Biology)
